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KMID : 0364519950070020193
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Dong-A Journal Medicine
1995 Volume.7 No. 2 p.193 ~ p.202
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Rapid Detection of the mec A Gene in Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction
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Abstract
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icillin-resistant Staphylococcus aureus (MRSA) is an increasingly common cause of nosocomial infections worldwide. Therefore, it is necessary that this resistance phenotype should be properly identified by clinical microbiology laboratories in
hospitals. Unfortunately, methicillin resistance of S. aureus is influenced by culture conditions such as temperature, incubation time, inoculum size and NaCI content in medium. These factors complicate the detection of methicillin resistance,
especially for the strains with heterogeneous resistance.
Among the 183 clinical isolates of S. aureus tested by the oxacillin broth dilution MIC test, 75 strains(41.0%) were resistant to oxacillin(MRSA) and 108 strains(59.0%) were susceptible to oxacillin (MSSA). In order to identify MRSA from clinical
sources with ease and reliability, a 533-bp fragment of the mec A gene was targeted for PCR. Results obtained by this method were compared with those obtained by oxacillin broth dilution MIC determination. Seventy three strains (97.3%) of 75 MRSA
and 4
strains(3.7%) of 108 MSSA were positive for mec A gene. The effects of various incubation conditions on oxacillin and methicillin disk diffusion tests were also compared. Five MRSA and 10 MSSA strains were showed the different results in
comparison
with
oxacillin MIC test.
These results suggest that mec A gene analysis by PCR may be useful in complementing exiting culture techniques for rapid and accurate determination of MRSA.
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KEYWORD
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